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Background: Obesity is a multifactorial disease, which occurs as a result of excessive fat tissue accumulation and if not treated properly in the long term will lead to risks and complications of dangerous diseases including diabetes mellitus, cardiovascular disease, reproductive system disorders, gallstones, and bone diseases and joints. The purpose of this study is to determine the effect of acupuncture on glucose metabolism in overweight and obese patients. Methods: This study uses a quasiexperimental one-group pretest-posttest design. This research was conducted at the Posyandu Elderly in Mojosongo, Jebres, Surakarta. This study focuses on acupuncture therapy on blood glucose levels, body mass index (BMI) and serum Hb.A1c levels before and after acupuncture therapy. Data analysis using Paired T-Test with SPSS Series 23 data processing application. Results: Showed that there was a significant but not significant effect of acupuncture on blood glucose levels in overweight and obese patients(ρ=0,524), there was a significant effect of acupuncture on Body Mass Index (BMI) in overweight and obese patients(ρ=0,019), and there is a significant effect of acupuncture on Hb.A1c levels in overweight and obese patients( ρ=0,000). Conclusion: This study shows that acupuncture therapy can affect glucose metabolism and reduce body mass index so as to prevent the onset of dangerous chronic diseases in the future.